The Effects of pH and Temperature on the Growth of Escherichia coli DH5a
نویسنده
چکیده
Objectives/Goals The purpose of this project was to study the growth of the bacteria Escherichia coli DH5a under a variety of different pH and temperature, in order to discover and approximate the optimal value at which the cell was able to increase in size and divide the quickest. Methods/Materials Material used for this experiment included Eschericha coli DH5a, flasks, cuvettes, test tubes, adjustable pipettes, disposable tips, 12N HCl, 10N NaOH, graded transfer pipette, safety goggles, gloves, LB medium, lab coat, electric pipette filler, autoclaver, pH meter, Beckman DU640 spectophotometer, shaking incubators (New Brunswick Scientific)
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